Journal: Journal of Inflammation (London, England)

Article Title: Mitochondria protective and anti-apoptotic effects of peripheral benzodiazepine receptor and its ligands on the treatment of asthma in vitro and vivo

doi: 10.1186/s12950-024-00383-0

Figure Lengend Snippet: Subcellular structures detected by TEM. a There existed a large number of mitochondria with the normal structures and the intact cristae in Control group. b Mitochondrial injury was severe in LPS group. c The PBR agonist Ro5-4864 alleviated LPS-induced mitochondrial injury. d The PBR antagonist PK 11,195 alleviated LPS-induced mitochondrial injury

Article Snippet: Midazolam (Jiangsu Enhua, China), Ro5-4864 (MedChemExpress, USA), PK 11,195 (MedChemExpress, USA) and Cyclosporine-A (MedChemExpress, USA) were diluted in the Keratinocyte Medium containing 0.1% (v/v%) DMSO, achieving final concentrations of 2 μM, 10 μM, 10 μM, and 10 μM, respectively.

Techniques: Control

Journal: Journal of Inflammation (London, England)

Article Title: Mitochondria protective and anti-apoptotic effects of peripheral benzodiazepine receptor and its ligands on the treatment of asthma in vitro and vivo

doi: 10.1186/s12950-024-00383-0

Figure Lengend Snippet: Inflammatory cells in BALF. Cell smears with Wright-Giemsa staining: The inflammatory cells were increased and aggregated on the BALF smear of OVA group, decreased and scattered after the treatment of the Dexamethasone. Both Ro5-4864 and PK 11,195 made a decrease and scatter of inflammatory cells on the BALF smear in OVA-treated mice. Cell counting: The total numbers of cells (10 6 /ml) in the BALF are presented as mean ± SD. * p = 0.001 < 0.05 vs. Control group. ** p = 0.001 < 0.05 vs. OVA group. Bar = 50 μm

Article Snippet: Midazolam (Jiangsu Enhua, China), Ro5-4864 (MedChemExpress, USA), PK 11,195 (MedChemExpress, USA) and Cyclosporine-A (MedChemExpress, USA) were diluted in the Keratinocyte Medium containing 0.1% (v/v%) DMSO, achieving final concentrations of 2 μM, 10 μM, 10 μM, and 10 μM, respectively.

Techniques: Staining, Cell Counting, Control

Journal: Journal of Inflammation (London, England)

Article Title: Mitochondria protective and anti-apoptotic effects of peripheral benzodiazepine receptor and its ligands on the treatment of asthma in vitro and vivo

doi: 10.1186/s12950-024-00383-0

Figure Lengend Snippet: Histopathological examination. The lungs histopathological examination showed the injury of BECs and the infiltration of inflammatory cells in the peribronchial and submucosal tissue spaces in OVA group. The injury of BECs and the infiltration of inflammatory cells were abated after the treatments of Ro5-4864, PK 11,195 and Dexamethasone

Article Snippet: Midazolam (Jiangsu Enhua, China), Ro5-4864 (MedChemExpress, USA), PK 11,195 (MedChemExpress, USA) and Cyclosporine-A (MedChemExpress, USA) were diluted in the Keratinocyte Medium containing 0.1% (v/v%) DMSO, achieving final concentrations of 2 μM, 10 μM, 10 μM, and 10 μM, respectively.

Techniques: